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KMID : 0369820120420010047
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Jorunal of Korean Pharmaceutical Sciences
2012 Volume.42 No. 1 p.47 ~ p.50
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Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography?ultraviolet (HPLC?UV) detection
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Lee Jin-Gyun
Shin Sang-Yeon
Shin Hae-Jin
Huh Yoon-Young
Lee Seul-Ji
Kim Dong-Hwan
Lee Su-Jung
Kim Young-Ok
Han Sang-Beom
Lee Jeong-Mi
Park Jeong-Hill
Kwon Sung-Won
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Abstract
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High performance liquid chromatography?ultraviolet (HPLC?UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC?UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation.
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KEYWORD
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Preservatives, Cresol, Chlorocresol, Benzethonium, HPLC?UV
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